Chemical characterization, antioxidant and antidiabetic activities of a novel polyherbal formulation comprising of Hordeum vulgare, Elettaria cardamomum and Cicer arietinum extracts

Diabetes mellitus (DM) is the most prevalent endocrine disorder. Numerous individual herbs possess anti-diabetic activity. The seeds of Hordeum vulgare, Elettaria cardamomum and Cicer arietinum are traditionally used to manage DM. ambition this work was formulate poly-herbal granules (PHGs) comprising these three functional foods evaluate their in-vitro antioxidant antidiabetic potential. dried seed extracts were in a ratio 2.5:1:1 PHGs by wet granulation method. selected on basis traditional phytotherapies popularly local Hakeems Pakistan achieve glycemic control diabetic patients resistant allopathic regime medicine. flow properties developed evaluated. UV–Visible spectroscopic, Fourier Transform Infrared (FTIR) HPLC-DAD all performed. DPPH, FRAP, total capacity (TAC) Nitric Oxide (NO) scavenging assays carried out PHGs. activity investigated alpha-amylase alpha-glucosidase enzyme inhibition exhibited excellent properties. UV–Vis spectra demonstrated peak at 278 nm showing presence flavonoids phenols. FTIR confirmed existence flavonoids, phenols along with amines as well test revealed gallic acid, ascorbic Quercetin-3-(caffeoyldiglucoside)-7-glucoside, Rosmarinic delphinidin-3,5-diglucosides, Kaempferol-3-feruloylsophoroside-7-glucoside Phloroglucinol IC50 51.23, 58.57, 55.41 53.13 μg/mL DPPH assay, FRAP TAC, oxide respectively. also 49.97 36.16 respectively dose dependent manner. an property. These exhibit significant profile virtue flavonoid phenolic acid derivatives.